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To explore the clinical association between NDC80 and MAD2L1 expression with LUAD and LUSC clinical features, we used two methods. Firstly, the clinical information of 482 lung squamous cell carcinoma (Detailed in Table S1) and 223 adenocarcinoma cases (Table S2) were downloaded from TCGA data (same information being used for COX regression analysis), and the results showed that NDC80 expression statistical significantly associates with LUAD patients age, smoking, and stage in adenocarcinoma, the gene tends to express higher in younger (

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In the study, different web-based dastsets were used for data analysis. The web links to all the original data sources were listed as below: Three cDNA expression files GSE44077 (based on GPL6244[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array. Web link: =GSE44077), GSE18842 (based on GPL570[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array. Web link: =GSE18842) and GSE33532 (based on GPL570[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array. Web link: =GSE33532) were downloaded from Gene Expression Omnibus (GEO). And during the survival analysis of genes, 223 lung adenocarcinoma and 482 lung squamous cell carcinoma data were obtained from The Cancer Genome Atlas Program (TCGA) (Detailed in Supplementary Table 1 and Supplementary Table 2), meanwhile another analysis was conducted based on UALCAN (an interactive web resource for analyzing cancer transcriptome data. Web link: ) provided lung adenocarcinoma and squamous cell carcinoma data. All data generated from the analysis process of this study are available from the corresponding author on reasonable request.

Since the number of samples cases being used for IHC experiment was relatively low (61 cases for adenocarcinoma and 46 for squamous cell carcinoma respectively), and the number of patients with greater than 2, 3, 4 and 5 years follow-up was 70, 22, 9 and 6 respectively, the median follow-up of the 107 cases was 30 months. To avoid the limitations of relatively small number samples and short duration of follow-up, greater data of NSCLC samples were downloaded from TCGA database, including the clinical and mRNA transcription information of 574 lung adenocarcinoma and 555 lung squamous cell carcinoma for multivariate regression analysis. 041b061a72


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